I have problem in sql with asp.net application

Dear All,

I have get the error run asp.net application

Timeout expired. The timeout period elapsed prior to obtaining a connection from the pool. This may have occurred because all pooled connections were in use and max pool size was reached

Line 41:
Line 42: Dim sqlcon As SqlConnection = New SqlConnection(GetConnectionString)
Line 43: sqlcon.Open()
Line 44:
Line 45: Dim sqlcom As SqlCommand

Please hep me.

Rathnayake