Allow only ASP.NET Machine account to access a file

Hi,

 

I want only ASP.NET machine account to read a file. I am using IIS 5. Also ASP.NET machine account is given read permission to the folder. I have given folder level read permission to folder & file. But I get the below error;

System.UnauthorizedAccessException: Access to the path "c:\Inetpub\wwwroot\TestProject\xxxx\xyz.txt" is denied.

Any suggestions please...
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