Error uploading file - Could not find a part of the path

I am having trouble uploading any file to the path in the error message below. Does anyone have any idea what the problem is. I did some research on this and most sites say that the permissions need Read/Write access for the asp.net user. I tried this and I still receiver the error. Here is the error:
 Error saving file education_calendar.pdf System.IO.DirectoryNotFoundException: Could not find a part of the path "d:\inetpub\aopha\Uploads\". at System.IO.__Error.
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