prevent blank space in email regular expression

Hi

I have a regular expression validation for a email, this works fine until a user enter a blank space, then it's validated at all. How can I prevent that?

ValidationExpression="^([a-zA-Z0-9_\-])+(\.([a-zA-Z0-9_\-])+)*@((\[(((([0-1])?([0-9])?[0-9])|(2[0-4][0-9])|(2[0-5][0-5])))\.(((([0-1])?([0-9])?[0-9])|(2[0-4][0-9])|(2[0-5][0-5])))\.(((([0-1])?([0-9])?[0-9])|(2[0-4][0-9])|(2[0-5][0-5])))\.(((([0-1])?([0-9])?[0-9])|(2[0-4][0-9])|(2[0-5][0-5]))\]))|((([a-zA-Z0-9])+(([\-])+([a-zA-Z0-9])+)*\.)+([a-zA-Z])+(([\-])+([a-zA-Z0-9])+)*))$"
...