How to uncheck the checkboxlist after checking the box (asp.net vb.net)

Hi there 
i would like to uncheck the checkboxlist after choosing the boxes and saving them to database , literally these boxes will be disable . and there is a limit : 5times / each check box
i also attached my code below , thanks in advance
table Seat : ID (int ) PK , SeatNumber (varchar(50) )null
the checkboxlist
 


here is my code
Imports System.Data Imports System.Data.SqlClient Imports System.Configuration Partial Class test12 Inherits System.Web.UI.Page Dim con As SqlConnection = New SqlConnection(ConfigurationManager.ConnectionStrings("ConnectionString2").ToString()) Protected Sub Page_Load(ByVal sender As Object, ByVal e As System.EventArgs) Handles Me.Load If con.State = ConnectionState.Closed Then con.Open() E ...